제품 상세
임상 및 환경 검체에 존재하는 Aeromonas hydrophila를 분리하기 위한 선택 감별 배지
Aeromonas Medium (Ryan)
Code: CM0833
Typical Formula* | gm/litre |
Proteose peptone | 5.0 |
Yeast extract | 3.0 |
L-Lysine monohydrochloride | 3.5 |
L-Arginine monohydrochloride | 2.0 |
Sorbitol | 3.0 |
Inositol | 2.5 |
Lactose | 1.5 |
Xylose | 3.75 |
Bile Salts No.3 | 3.0 |
Sodium thiosulphate | 10.67 |
Sodium chloride | 5.0 |
Ferric ammonium citrate | 0.8 |
Bromothymol blue | 0.04 |
Thymol blue | 0.04 |
Agar | 12.5 |
Final pH 8.0 + 0.2 @ 25°C | |
* Adjusted as required to meet performance standards
AMPICILLIN SELECTIVE SUPPLEMENT
Code: SR0136
Vial contents (each vial is sufficient for 500 ml of medium) | per vial | per litre |
Ampicillin | 2.5mg | 5.0mg |
Directions
Suspend 29.5 g in 500ml of distilled water. Bring gently to the boil. DO NOT AUTOCLAVE. Cool to 50°C and aseptically add one vial of Ampicillin Selective Supplement SR0136 reconsituted as directed. Mix well and pour plates.
Appearance
Dehydrated medium: Straw coloured, free-flowing powder
Prepared medium: Green coloured gel
Quality control
Positive control: | Expected results |
Aeromonas hydrophila ATCC® 7966* | Good growth; opaque green colonies with dark centres |
Negative control: |
|
Escherichia coli ATCC® 11775* | No growth |
* This organism is available as a Culti-Loop®
Precautions
Although Aeromonas and Plesiomonas spp will grow on the medium if ampicillin is omitted, it will be more difficult to distinguish them from the other organisms present on the plate. Suspected colonies of Aeromonas spp must be confirmed by biochemical tests.